EMPIAR-12436 - Single Particle cryo-EM dataset of the shaped pulse revitrified 50S ribosomal subunit [7725 multi-frame micrographs composed of 45 frames each in TIFF format]

EMPIAR-12436

Single Particle cryo-EM dataset of the shaped pulse revitrified 50S ribosomal subunit [7725 multi-frame micrographs composed of 45 frames each in TIFF format]

Publication:

Laser flash melting cryo-EM samples to overcome preferred orientation

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ

Nature methods 22 (2025) 1880-1886

PMID: 40877464

DOI: 10.1038/s41592-025-02796-y

Related EMDB entry:

EMD-51751

Deposited:

2024-11-04

Released:

2025-07-25

Last modified:

2025-07-25

Imageset size:

1.08 TB

Imageset DOI:

10.6019/EMPIAR-12436

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw micrographs of shaped pulse revitrified 50S ribosomal subunit

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7725

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.651, 0.651)

Details:

Movies were collected in super-resolution mode (0.3255 A) and binned on the fly to the pixel size of 0.651 A

Files:

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)