EMPIAR-12411 - Cryo-EM structure of F1-like ATPase for the gliding motility of Mycoplasma [7350 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12411

Cryo-EM structure of F1-like ATPase for the gliding motility of Mycoplasma [7350 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Dimeric assembly of F<sub>1</sub>-like ATPase for the gliding motility of <i>Mycoplasma</i>

Toyonaga T, Kato T, Kawamoto A, Miyata T, Kawakami K, Fujita J, Hamaguchi T, Namba K, Miyata M

Science advances 11 (2025)

PMID: 40009674

DOI: 10.1126/sciadv.adr9319

Related EMDB entry:

EMD-60718

Deposited:

2024-09-19

Released:

2025-06-19

Last modified:

2025-06-19

Imageset size:

1.56 TB

Imageset DOI:

10.6019/EMPIAR-12411

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Mycoplasma F1-like ATPase

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7350

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.87, 0.87)

Details:

For gain references, use "gain.mrc" instead of the one listed in the header.
(no flipping and rotation)
- Cs: 2.7mm
- HT: 300 kV
- Pixel size: 0.87 Å/pix (If the values listed in the header are different, they should be noted.)
- Dose per frame: 2 electron/Å^2

Details
Approximately 500 images were included in the edges of the electron beam because the electron beam was shifted during the collecting dataset. These images were removed from the analysis.

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Citations

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Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)