For gain references, use "gain.mrc" instead of the one listed in the header. (no flipping and rotation) - Cs: 2.7mm - HT: 300 kV - Pixel size: 0.87 Å/pix (If the values listed in the header are different, they should be noted.) - Dose per frame: 2 electron/Å^2
Details Approximately 500 images were included in the edges of the electron beam because the electron beam was shifted during the collecting dataset. These images were removed from the analysis.