EMPIAR-12324 - CryoEM of E. coli RNA polymerase on lambda PR promoter DNA [3511 multi-frame micrographs composed of 30 frames each in TIFF format]

EMPIAR-12324

CryoEM of E. coli RNA polymerase on lambda PR promoter DNA [3511 multi-frame micrographs composed of 30 frames each in TIFF format]

Publication:

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 34599106

DOI: 10.1073/pnas.2112877118

Related EMDB entry:

EMD-23893 EMD-23892

Deposited:

2024-10-04

Released:

2024-12-05

Last modified:

2024-12-05

Imageset size:

967.95 GB

Imageset DOI:

10.6019/EMPIAR-12324

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3511

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.06, 1.06)

Details:

FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.70 e-/ A^2/s; frame time 0.2 s (30 frames) with total exposure of 6 s; total dose 46.2 e-/A^2; pixel size 1.06 A/px(detector); pixel size 0.53 A/px(image); defocus range -0.9 to -2.4 um; gain reference 18jul13c_17105458_05_7676x7420_norm_0_mod.mrc in references folder.

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Citations

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He L, Bartesaghi A. (2026)

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Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

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Jones HN, Deshmukh A, Pande K. (2026)

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Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)