EMPIAR-12323 - Unaligned multi-frame micrographs of the mu opioid receptor bound to a nanobody antagonist NbE [5939 multi-frame micrographs composed of 44 frames each in MRC format]

EMPIAR-12323

Unaligned multi-frame micrographs of the mu opioid receptor bound to a nanobody antagonist NbE [5939 multi-frame micrographs composed of 44 frames each in MRC format]

Publication:

Structural basis of μ-opioid receptor targeting by a nanobody antagonist

Yu J, Kumar A, Zhang X, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M

Nature communications 15 (2024)

PMID: 39384768

DOI: 10.1038/s41467-024-52947-6

Related EMDB entry:

EMD-18541

Deposited:

2024-09-04

Released:

2025-01-09

Last modified:

2025-01-09

Imageset size:

7.97 TB

Imageset DOI:

10.6019/EMPIAR-12323

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the µ-opioid receptor bound to a nanobody antagonist NbE

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

5939

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.9759, 0.9759)

Details:

1. All the images are raw data.

2. All the images are multi-frame micrographs composed of 44 frames each in MRC format.

3. All the images are acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV using a Falcon III direct electron detector at a nominal
magnification of 150,000x, resulting in a pixel size of 0.9759 Å.

4. Data were collected using EPU with a defocus range of −0.6 to −2.0 μm and a total electron dose of 40 e−/Å2

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