Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM
Voltage 300 keV Camera Gatan K3, super-resolution mode with 2-fold binning by EPU Energy filter GIF, 20eV slit width Magnification 130000 Pixel size 0.663 A/pix Dose 40 e/A2 Exposure rate 19.9 e/pix/s Exposure time 0.9 s Fractions 40
Total mics 14220 (grid 1) 13991 (grid 2)
Each grid has its own "gainref.mrc" gain reference file. These weres binned using 'rebin by two' in Digital Micrograph. They need to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can be used as is for cryoSPARC.
particles_resting.star contains metadata for the 230,001 particles refined for the resting state (EMDB 45085).
Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM
Voltage 300 keV Camera Gatan K3, super-resolution mode with 2-fold binning by EPU Energy filter GIF, 20eV slit width Magnification 130000 Pixel size 0.663 A/pix Dose 40 e/A2 Exposure rate 20.4 Exposure time 0.87 s Fractions 40
Total mics 18453
Each grid has its own "gainref.mrc" gain reference file. These were binned using 'rebin by two' in Digital Micrograph. They need to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can be used as is for cryoSPARC.
particles_elongating.star contains metadata for the 136,601 particles refined for the elongating state (EMDB 45086).
