Single-particle cryo-EM structure of the RNA chaperone Hfq in a translation repression complex Hfq-Crc-estA [13651 multi-frame micrographs composed of 47 frames each in TIFF format]
Publication:
Translational regulation by Hfq-Crc assemblies emerges from polymorphic ribonucleoprotein folding
Hfq/Crc translation repression complex formed on estA(118) present in micrographs. The images were taken using an FEI Titan Krios G2, 300kV, with an average electron dose per frame of 1.02 electrons per square Angstrom. Defocus -1.1 to -2.5um.