Micrographs were obtained from movie-stacks of 20 frames à 2-2.5 e/A^2 by global motion-correction and dose-weighting using Relion 4.0, CTF parameters were estimated with GCTF 1.06 and particles were selected by GAUTOMATCH 0.56. - Micrographs star-file (Micrographs with FinalResolution better 4.5A): data/corrected_micrographs.star - Particle coordinates: data/corrected_micrographs/*_automatch.star
Star-files for individual particle populations are located in data/particles (see below), masks used for sorting and final refinements in data/particles/masks360. Star-file for all shiny particles: - data/shiny_particles360_all.star Star-files for shiny particles of major states - data/particles/shiny_particles360_48S-1.star - data/particles/shiny_particles360_48S-2.star - data/particles/shiny_particles360_48S-3.star - data/particles/shiny_particles360_48S-4.star - data/particles/shiny_particles360_48S-5.star Star-files for shiny particles of eIF2, tRNA and eIFB5 substates: - data/particles/shiny_particles360_48S-2-1.star - data/particles/shiny_particles360_48S-2-2.star - data/particles/shiny_particles360_48S-2-3.star - data/particles/shiny_particles360_48S-3-1.star - data/particles/shiny_particles360_48S-3-2.star - data/particles/shiny_particles360_48S-3-3.star - data/particles/shiny_particles360_48S-4-1.star - data/particles/shiny_particles360_48S-4-2.star - data/particles/shiny_particles360_48S-5-1.star - data/particles/shiny_particles360_48S-5-2.star - data/particles/shiny_particles360_48S-5-3.star Star-files for shiny particles of eIF3bi substates: - data/particles/shiny_particles360_48S-1_eIF3bi_majorstate.star - data/particles/shiny_particles360_closed48S_eIF3bi_majorstate.star - data/particles/shiny_particles360_closed48S_eIF3bi_minorstate1.star - data/particles/shiny_particles360_closed48S_eIF3bi_minorstate2.star
Star-files with subtracted particles used for local refinement of eIF3core in open 48S (48S-1): - data/subtracted_particles/subtracted_particles240_eIF3core_open48S.star Masks used for refinements are located in data/subtracted_particles/masks240.
Star-files with subtracted particles used for local refinement of eIF3core in closed 48S (48S-2 to 5): - data/subtracted_particles/subtracted_particles240_eIF3core_closed48S.star Masks used for refinements are located in data/subtracted_particles/masks240.