EMPIAR-12067
Single-particle cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA [6271 multi-frame micrographs composed of 38 frames each in TIFF format]
Publication:

A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

Dendooven T, Sinha D, Roeselová A, Cameron TA, De Lay NR, Luisi BF, Bandyra KJ

Molecular cell 81 (2021) 2901-2913.e5

PMID: 34157309

Related EMDB entry:
Deposited:
2024-03-20
Released:
2024-06-12
Last modified:
2024-06-12
Imageset size:
6.78 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6271
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 32 BIT INTEGER
Pixel spacing:
(1.065, 1.065)
Details:
PNPase-3'ETS(LeuZ)_Hfq present in micrographs. The images were taken using an FEI Titan Krios, 300kV, with an average electron dose per image of 53.6 electrons per square Angstrom. Defocus -1 to -2.5um.
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