EMPIAR-12067 - Single-particle cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA [6271 multi-frame micrographs composed of 38 frames each in TIFF format]

EMPIAR-12067

Single-particle cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA [6271 multi-frame micrographs composed of 38 frames each in TIFF format]

Publication:

A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

Dendooven T, Sinha D, Roeselová A, Cameron TA, De Lay NR, Luisi BF, Bandyra KJ

Molecular cell 81 (2021) 2901-2913.e5

PMID: 34157309

DOI: 10.1016/j.molcel.2021.05.032

Related EMDB entry:

EMD-12884

Deposited:

2024-03-20

Released:

2024-06-12

Last modified:

2024-06-12

Imageset size:

6.78 TB

Imageset DOI:

10.6019/EMPIAR-12067

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6271

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.065, 1.065)

Details:

PNPase-3'ETS(LeuZ)_Hfq present in micrographs. The images were taken using an FEI Titan Krios, 300kV, with an average electron dose per image of 53.6 electrons per square Angstrom. Defocus -1 to -2.5um.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)