Cryo-electron tomography of mitotic chromosomes isolated from chicken DT-40 cells with annotated nucleosome positions [multiple data sets in TIFF, MRCS and MRC formats]
Publication:
Template Learning: Deep learning with domain randomization for particle picking in cryo-electron tomography
Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks using Motioncor2. The tilt series were reconstructed using etomo (IMOD). Original metadata files (.mdoc) are provided in the mdoc folder and tomograms reconstructed using etomo (IMOD) (.mrc file in Tomogram folder). The alignment information (.xf file) is in the Alignment_info folder.
Tomogram (4-times binned reconstructions, original pixel size 2.075 Å) from aligned tilt series (.mrcs file in TiltSeries folder). The coordinates of nucleosomes annotated by Template Learning and template matching are in the picks folder.