FIB-SEM dataset showing localization of a Golgi matrix protein GM130 in human hepatocellular carcinoma cell (Huh-7) [564 reconstructed volumes in TIFF format]
Publication:
Localization of a Golgi matrix protein GM130 in human hepatocellular carcinoma cell (Huh-7)
Huh7, a human hepatoma-derived cell was immunostained using antibody against Golgi matrix protein GM130 and imaged using Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) with voxels of 4.0 x 4.0 x 8.0 nm.
Immunolabeling as described in Lak B, Li S, Belevich I, Sree S, Butkovic R, Ikonen E, Jokitalo E. Specific subdomain localization of ER resident proteins and membrane contact sites resolved by electron microscopy. Eur J Cell Biol. 2021 Sep-Nov;100(7-8):151180. doi: 10.1016/j.ejcb.2021.151180. Epub 2021 Oct 4
Stained using ferrocyanide-reduced osmium + thiocarbohydrazide + osmium (OTO) method
Purified mouse anti-GM130 was from BD biosciences.
