Automethylated PRC2 dimer bound to nucleosome [multiple data sets in MRC format]
Publication:
Activation of automethylated PRC2 by dimerization on chromatin
Sauer PV, Pavlenko E, Cookis T, Zirden LC, Renn J, Singhal A, Hunold P, Hoehne-Wiechmann MN, van Ray O, Kaschani F, Kaiser M, Hänsel-Hertsch R, Sanbonmatsu KY, Nogales E, Poepsel S
The sample used here was PRC2 (EZH2, SUZ12,EED, RBAP48, AEBP2, but without Jarid2) together with H1.0 bound to nucleosome. However it didn't form a complex. Instead, this dataset yielded a structure of H1.0 bound to nucleosome alone. In addition, the quality of the streptavidin lattice was not good, so the subtraction was incomplete. Therefore, when processing this dataset there might be leftovers of streptavidin appearing in the 2D classes. This dataset was recorded on a Talos Arctica at 200kV with a dose of 50 electrons per square Angstrom.