Gain reference file for cryoSPARC is located in: data/11162022_iversot_singhpk_MSC-ring_68-12-1_EPU/downsized_gain_ref.mrc Following are parameters for CryoSPARC 4.2.1: Rotate gain ref? = 2 Raw pixel size (A) = 0.818 Spherical Aberration (mm) = 2.7 Total exposure dose (e/A^2) = 54.368