EMPIAR-11538
Cryo electron tomography - tilt-series of FIB-milled HEK293T cells under untreated and HHT-treated conditions [710 tilt series in MRC format]
Publication:

Translation dynamics in human cells visualized at high-resolution reveal cancer drug action

Xing H, Welsch S, Turoňová B, Beck M

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Related EMDB entry:
Deposited:
2023-04-27
Released:
2024-04-30
Last modified:
2024-04-30
Imageset size:
1.26 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
1. Tilt-series of FIB-milled HEK293T cells under native and HHT-treated conditions
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
710
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.188, 1.188)
Details:
All mdoc files corresponding to the tilt-series are provided. Tilt series were acquired on a Titan Krios G4 (Thermo Fisher Scientific) operated at 300kV and equipped with Selectris X imaging filter and Falcon 4 direct electron detector, at 4K x 4K pixel dimensions, tilt range of -60° to 60°, 2° tilt increment, target defocus of -1.5 to -4.5 μm and a total dose of 120 to 150 e/Å2 per tilt series, using SerialEM software. There are 30-61 tilts per tilt series, which depends on when the tilt series data collection finished or failed. Target areas were selected in the cytoplasmic region of the cell.
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)
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