EMPIAR-11538
Cryo electron tomography - tilt-series of FIB-milled HEK293T cells under untreated and HHT-treated conditions [710 tilt series in MRC format]
Publication:

Translation dynamics in human cells visualized at high-resolution reveal cancer drug action

Xing H, Welsch S, Turoňová B, Beck M

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Related EMDB entry:
Deposited:
2023-04-27
Released:
2024-04-30
Last modified:
2024-04-30
Imageset size:
1.26 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
1. Tilt-series of FIB-milled HEK293T cells under native and HHT-treated conditions
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
710
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.188, 1.188)
Details:
All mdoc files corresponding to the tilt-series are provided. Tilt series were acquired on a Titan Krios G4 (Thermo Fisher Scientific) operated at 300kV and equipped with Selectris X imaging filter and Falcon 4 direct electron detector, at 4K x 4K pixel dimensions, tilt range of -60° to 60°, 2° tilt increment, target defocus of -1.5 to -4.5 μm and a total dose of 120 to 150 e/Å2 per tilt series, using SerialEM software. There are 30-61 tilts per tilt series, which depends on when the tilt series data collection finished or failed. Target areas were selected in the cytoplasmic region of the cell.
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