EMPIAR-11530
Single particle cryo-EM dataset of Mus musculus mitochondrial complex I bound with inhibitor Piericidin A [1200 multi-frame micrographs composed of 25 frames each in MRC format]
Publication:

Investigation of hydrated channels and proton pathways in a high-resolution cryo-EM structure of mammalian complex I

Grba DN, Chung I, Bridges HR, Agip AA, Hirst J

Science advances 9 (2023)

PMID: 37531432

Related EMDB entry:
Deposited:
2023-04-24
Released:
2023-08-18
Last modified:
2023-08-18
Imageset size:
1.55 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of wild-type mouse mitochondrial complex I from an as-prepared inhibited by piericidin A
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1200
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.05, 1.05)
Details:
Gain-corrected micrographs were imaged using a Gatan K2 detector in counting mode with a GIF BioQuantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. The sample was frozen on PEGylated gold grids.
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