EMPIAR-11481 - Movies of GroEL-ES-nucleotide complex plunged 13, 50, 200 ms and 20 s after mixing with ATP [multiple data sets in TIFF format]

EMPIAR-11481

Movies of GroEL-ES-nucleotide complex plunged 13, 50, 200 ms and 20 s after mixing with ATP [multiple data sets in TIFF format]

Publication:

Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting

Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG

Nature Methods (---)

DOI: 10.1038/s41592-023-01967-z

Related EMDB entry:

EMD-16117 EMD-16106 EMD-16119 EMD-16115 EMD-16108 EMD-16154 EMD-16116 EMD-16118 EMD-16100 EMD-16099 EMD-16107 EMD-16125 EMD-16102 EMD-16109

Deposited:

2023-02-08

Released:

2023-08-18

Last modified:

2023-08-18

Imageset size:

2.94 TB

Imageset DOI:

10.6019/EMPIAR-11481

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. GroELES with 20s of mixing time

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4194

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 20sGroEL-ATP.star, 20sGroEL-ATP-GroES.star, 20sGroEL-ATP-ADP-GroES.star and 20s_GroEL-ATP-2GroES.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

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rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. GroELES at 50ms timepoint

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1416

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 50ms_GroEL-ADP.star, 50ms_GroEL-ATP-ADP.star, 50ms_GroEL-ATP.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrcGroEL was mixed with GroES and ATP and plunged within 50ms

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. GroEL 13ms timepoint

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2609

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 13ms_GroEL-ADP.star, 13ms-GroEL-ATP-ADP.star, 13ms_GroEL-ATP.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. GroELES 200ms timepoint

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5445

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

STAR files associated with the reconstructed maps from this dataset are uploaded in the directory 'associated_star_files' with names 200msGroEL-ATP.star, 200msGroEL-ATP-GroES.star, 200msGroEL-ATP-ADP-GroES.star and 200ms_GroEL-ATP-2GroES.star

gain files are included in the Gain file directory under particular time point. Please use the corresponding gain file by matching the gain file date and micrograph date.
The dates are in format YYYYMMDD
The date is indicated at the start of micrograph name e.g 20220628_trGroEL...tif
The date is the second part of gain refence file name e.g CountRef_20220628_trGroEL...mrc

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）