EMPIAR-11472
Single particle cryo-EM dataset of human nucleolar and nuclear pre-60S assembly intermediates [multiple data sets in TIFF and MRCS formats]
Publication:

Principles of human pre-60S biogenesis

Vanden Broeck A, Klinge S

Science (New York, N.Y.) 381 (2023)

PMID: 37410842

Deposited:
2022-12-06
Released:
2023-09-26
Last modified:
2023-09-26
Imageset size:
140.46 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
44352
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
Files:
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2. Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
45846
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
Files:
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3. Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 3
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
44335
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
Files:
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4. Unaligned multi-frame micrographs of human nucleolar and nuclear pre-60S assembly intermediates - Dataset 4
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
37166
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.
Files:
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5. Polished particle stack of human nucleolar and nuclear pre-60S assembly intermediates
Category:
micrographs - single frame
Image format:
MRCS
No. of images or tilt series:
2474967
Image size:
(480, 480)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.08, 1.08)
Details:
Merged set of pre-60S particles from four datasets. Particles are polished, magnification anisotropy corrected and per-particle defocus corrected. A Relion .star format file is provided and contains the final Euler angles, shifts and also the micrograph coordinates.
Files:
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