EMPIAR-11453
Cryo electron tomography of induced protrusions of cofilin or control knock-down Drosophila S2 cells - Datasets 9 - 12 [multiple data sets in TIFF and MRC formats]
Publication:

CryoET shows cofilactin filaments inside the microtubule lumen

Ventura Santos C, Carter AP, Rogers SL

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Related EMDB entry:
Deposited:
2023-03-07
Released:
2023-07-10
Last modified:
2023-07-10
Imageset size:
491.89 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • reconstructed volumes
1. Dataset 9 part 1: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
28
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(2.659, 2.659)
Details:
Data from Dataset 9 part 1: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference is provided within the main folder. Data was collected on a K3 camera at a pixel size of 2.659 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.
TS_330 - TS_351 are from cofilin knock-down cells. TS_352 - TS_366 are from control knock-down cells.
Files:
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2. Dataset 9 part 1: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
28
Image size:
(920, 1298)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.81, 11.81)
Details:
Dataset 9 part 1: Tomograms binned to 11.81 Å/ pixel, reconstructed and deconvolved in WARP.
TS_330 - TS_351 are from cofilin knock-down cells. TS_352 - TS_366 are from control knock-down cells.
Files:
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3. Dataset 9 part 2: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
8
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 9 part 2: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.
TS_462 - TS_470 are from control knock-down cells.
Files:
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4. Dataset 9 part 2: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
8
Image size:
(960, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.81, 11.81)
Details:
Dataset 9 part 2: Tomograms binned by 4 (11.81 Å/ pixel), reconstructed and deconvolved in WARP.
TS_462 - TS_470 are from control knock-down cells.
Files:
Loading...
5. Dataset 10: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
34
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 10: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.
TS_424 - TS_442 are from cofilin knock-down cells. TS_443 - TS_461 are from control knock-down cells.
Files:
Loading...
6. Dataset 10: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
34
Image size:
(960, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.81, 11.81)
Details:
Dataset 10: Tomograms binned by 4 (11.81 Å/ pixel), reconstructed and deconvolved in WARP.
TS_424 - TS_442 are from cofilin knock-down cells. TS_443 - TS_461 are from control knock-down cells.
Files:
Loading...
7. Dataset 11 part 1: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
32
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 11 part 1: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.
TS_495 - TS_505 are from cofilin knock-down cells. TS_506 - TS_527 are from control knock-down cells.
Files:
Loading...
8. Dataset 11 part 1: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
32
Image size:
(960, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.8, 11.8)
Details:
Dataset 11 part 1: Tomograms binned by 4 (11.8 Å/ pixel), reconstructed and deconvolved in WARP.
TS_495 - TS_505 are from cofilin knock-down cells. TS_506 - TS_527 are from control knock-down cells.
Files:
Loading...
9. Dataset 11 part 2: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(2.659, 2.659)
Details:
Data from Dataset 11 part 2: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference is provided within the main folder. Data was collected on a K3 camera at a pixel size of 2.659 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.
TS_367, TS_371, TS_380 are from cofilin knock-down cells.
Files:
Loading...
10. Dataset 11 part 2: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
3
Image size:
(1022, 1440)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(10.64, 10.64)
Details:
Dataset 11 part 2: Tomograms binned by 4 (10.64 Å/ pixel), reconstructed and deconvolved in WARP.
TS_367, TS_371, TS_380 are from cofilin knock-down cells.
Files:
Loading...
11. Dataset 12: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
27
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.952, 2.952)
Details:
Data from Dataset 12: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder.
TS_528 - TS_540 are from cofilin knock-down cells. TS_542 - TS_556 are from control knock-down cells.
Files:
Loading...
12. Dataset 12: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
27
Image size:
(960, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(11.8, 11.8)
Details:
Dataset 12: Tomograms binned by 4 (11.8 Å/ pixel), reconstructed and deconvolved in WARP.
TS_528 - TS_540 are from cofilin knock-down cells. TS_542 - TS_556 are from control knock-down cells.
Files:
Loading...
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