Arctica operated at 200 kV with a GatanK3 imaging system collected at 45,000X nominal magnification. The calibrated pixel size of 0.4296 Å was used for processing. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 33.42 e-/Å2/s with a total exposure of 1.60 seconds, for an accumulated dose of 53.47 e-/Å2. Intermediate frames were recorded every 0.04 seconds for a total of 40 frames per micrograph.
Arctica operated at 200 kV with a GatanK3 imaging system collected at 45,000X nominal magnification. The calibrated pixel size of 0.4296 Å was used for processing. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 33.78 e-/Å2/s with a total exposure of 1.60 seconds, for an accumulated dose of 54.04 e-/Å2. Intermediate frames were recorded every 0.04 seconds for a total of 40 frames per micrograph.