The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 0.86 Å/px. The dose rate is 0.90 e/Å2/frame. Please use the gain.tiff file a gain reference without any rotation or flipping.