micrographs - multiframe

TIFF

1619

(11520, 8184)

UNSIGNED BYTE

(0.4495, 0.4495)

Data collected on 11/23/20-11/24/20
Krios, K3, counted mode, EFTEM, Cs corrector, super-resolution
User/Group:Brian Caldwell/Chuck Bell

Image information:
Total # of images = 1633 selected, 22 grid squares
Total data size = 0.933 TB
Rate of collection = 69/hour
Mode of collection = Accurate hole centering
Gain normalized = NO
File format = LZW tiff
Super-resolution = YES
Spherical aberration = 0.01 mm
Images per hole = 1
Exposure time = 2.83 s
Fractions = 45

Microscope parameters:
Pixel size = 0.899 A
Magnification = 81,000
Stage tilt = 0 deg
Dose rate = 18.43 e/px/s
Dose rate = 22.80 e/A^2/s
Total dose = 65 e/A^2 [desired dose rate]
Defocus range = -3.5, -3, -2.5, -2, -1.5, -1
C2 aperture = 50 um
Objective aperture= 100 um
Illuminated area = 1.5 um
Energy Filter = YES
Slit width = 20 eV

Grid type = Quantifoil (gold coated) R1.2/1.3 300 Mesh hole size, mesh, etc.
Position 1 of the large dewar Bell Conical: Position 1 of box F2 (Purple)
Prepared on [date] by Brian Caldwell

Additional notes:
Copied gain reference file over into the data folder.
Gain reference image located at: /data/gain.mrc
Saved EPU settings file.

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