Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 1.076 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.
Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9953 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.
Contains polished particles (437,174) as 11,609 mrcs files from the pooled GDN samples (75 and 150 mM KCl). The selections of particles for each conformation (1 and 2) are in the main data folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.