EMPIAR-11140
Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the cingulate cortex of an individual with Parkinson's disease dementia -synuclein filaments [10874 multi-frame micrographs composed of 42 frames each in TIFF format]
Publication:

Structures of α-synuclein filaments from human brains with Lewy pathology

Yang Y, Shi Y, Schweighauser M, Zhang X, Kotecha A, Murzin AG, Garringer HJ, Cullinane PW, Saito Y, Foroud T, Warner TT, Hasegawa K, Vidal R, Murayama S, Revesz T, Ghetti B, Hasegawa M, Lashley T, Scheres SHW, Goedert M

Nature 610 (2022) 791-795

PMID: 36108674

Related EMDB entry:
Deposited:
2022-06-28
Released:
2022-11-11
Last modified:
2022-11-11
Imageset size:
1.17 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Cryo-EM reconstruction of alpha-synuclein filaments from Parkinson's disease dementia
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10874
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.727, 0.727)
Details:
The files were written as eer files by EPU and then converted to TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame for Dataset1 and Dataset2. Please use gain-reference accordingly without any rotation or flipping.
picking contains start-end coordinates of the manually picked filaments of Dataset1 and Dataset2, respectively.
Refine070_run_data.star contains STAR file(s) with the metadata of the final refined segments.
Files:
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