EMPIAR-11140 - Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the cingulate cortex of an individual with Parkinson's disease dementia -synuclein filaments [10874 multi-frame micrographs composed of 42 frames each in TIFF format]

EMPIAR-11140

Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the cingulate cortex of an individual with Parkinson's disease dementia -synuclein filaments [10874 multi-frame micrographs composed of 42 frames each in TIFF format]

Publication:

Structures of α-synuclein filaments from human brains with Lewy pathology

Yang Y, Shi Y, Schweighauser M, Zhang X, Kotecha A, Murzin AG, Garringer HJ, Cullinane PW, Saito Y, Foroud T, Warner TT, Hasegawa K, Vidal R, Murayama S, Revesz T, Ghetti B, Hasegawa M, Lashley T, Scheres SHW, Goedert M

Nature 610 (2022) 791-795

PMID: 36108674

DOI: 10.1038/s41586-022-05319-3

Related EMDB entry:

EMD-15285

Deposited:

2022-06-28

Released:

2022-11-11

Last modified:

2022-11-11

Imageset size:

1.17 TB

Imageset DOI:

10.6019/EMPIAR-11140

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Cryo-EM reconstruction of alpha-synuclein filaments from Parkinson's disease dementia

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10874

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.727, 0.727)

Details:

The files were written as eer files by EPU and then converted to TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame for Dataset1 and Dataset2. Please use gain-reference accordingly without any rotation or flipping.
picking contains start-end coordinates of the manually picked filaments of Dataset1 and Dataset2, respectively.
Refine070_run_data.star contains STAR file(s) with the metadata of the final refined segments.

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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