EMPIAR-11139
Cryo-EM structure of the human GS-GN complex in the inhibited state [multiple data sets in EER and MRC formats]
Publication:

Mechanism of glycogen synthase inactivation and interaction with glycogenin

Marr L, Biswas D, Daly LA, Browning C, Vial SCM, Maskell DP, Hudson C, Bertrand JA, Pollard J, Ranson NA, Khatter H, Eyers CE, Sakamoto K, Zeqiraj E

Nature communications 13 (2022)

PMID: 35690592

Related EMDB entry:
Deposited:
2022-03-23
Released:
2022-08-16
Last modified:
2022-08-18
Imageset size:
9.24 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multiframe movies in the EER format of the human glycogen synthase-glycogenin complex
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
20841
Image size:
(None, None)
Pixel type:
BIT
Pixel spacing:
(0.71, 0.71)
Details:
A dose per physical pixel/s of 4.71 was used resulting in a total dose of 34.8 e/Å2, fractionated across 128 EPU frames. This was then grouped into 21 frames, resulting in a dose per frame of 0.8 e/Å2. Micrograph file pattern: data/Images-Disc1/GridSquare_*/Data/*.eer
Files:
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2. Aligned micrographs of the human glycogen synthase-glycogenin complex
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
20841
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.71, 0.71)
Details:
Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 21. Micrograph file pattern: data/job008/Raw_data/*.mrc
Files:
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