EMPIAR-11139 - Cryo-EM structure of the human GS-GN complex in the inhibited state [multiple data sets in EER and MRC formats]

EMPIAR-11139

Cryo-EM structure of the human GS-GN complex in the inhibited state [multiple data sets in EER and MRC formats]

Publication:

Mechanism of glycogen synthase inactivation and interaction with glycogenin

Marr L, Biswas D, Daly LA, Browning C, Vial SCM, Maskell DP, Hudson C, Bertrand JA, Pollard J, Ranson NA, Khatter H, Eyers CE, Sakamoto K, Zeqiraj E

Nature communications 13 (2022)

PMID: 35690592

DOI: 10.1038/s41467-022-31109-6

Related EMDB entry:

EMD-14587

Deposited:

2022-03-23

Released:

2022-08-16

Last modified:

2022-08-18

Imageset size:

9.24 TB

Imageset DOI:

10.6019/EMPIAR-11139

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multiframe movies in the EER format of the human glycogen synthase-glycogenin complex

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

20841

Image size:

(None, None)

Pixel type:

BIT

Pixel spacing:

(0.71, 0.71)

Details:

A dose per physical pixel/s of 4.71 was used resulting in a total dose of 34.8 e/Å2, fractionated across 128 EPU frames. This was then grouped into 21 frames, resulting in a dose per frame of 0.8 e/Å2. Micrograph file pattern: data/Images-Disc1/GridSquare_*/Data/*.eer

Files:

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2. Aligned micrographs of the human glycogen synthase-glycogenin complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

20841

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.71, 0.71)

Details:

Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 21. Micrograph file pattern: data/job008/Raw_data/*.mrc

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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