EMPIAR-11076 - Single particle cryo-EM dataset of the Paranosema locustae ribosome bound to Lso2 [multiple data sets in MRC format]

EMPIAR-11076

Single particle cryo-EM dataset of the Paranosema locustae ribosome bound to Lso2 [multiple data sets in MRC format]

Publication:

Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome

Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, Barandun J

PLoS biology 18 (2020)

PMID: 33125369

DOI: 10.1371/journal.pbio.3000958

Related EMDB entry:

EMD-11437

Deposited:

2021-09-02

Released:

2022-08-09

Last modified:

2022-08-09

Imageset size:

1.96 TB

Imageset DOI:

10.6019/EMPIAR-11076

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the Paranosema locustae Lso2-ribosome complex - 20 frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3078

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.041, 1.041)

Details:

Micrographs were collected at the Umeå Core Facility for Electron Microscopy on a Titan Krios (Thermo Fisher Scientific) operated at 300 kV, equipped with a Gatan K2 BioQuantum direct electron detector. EPU (Thermo Fisher Scientific) was used for the automated data collection of a total of 3078 movies with 20 frames at a total
dose of 28.6 e−/Å2, a pixel size of 1.041 Å, and a defocus range between 0.7 and 2 μm.

Files:

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned multi-frame micrographs of the Paranosema locustae Lso2-ribosome complex - 40 frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2254

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.041, 1.041)

Details:

Micrographs were collected at the Umeå Core Facility for Electron Microscopy on a Titan Krios (Thermo Fisher Scientific) operated at 300 kV, equipped with a Gatan K2 BioQuantum direct electron detector. EPU (Thermo Fisher Scientific) was used for the automated data collection of a total of 2254 movies with 40 frames at a total
dose of 28.6 e−/Å2, a pixel size of 1.041 Å, and a defocus range between 0.7 and 2 μm.

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）