EMPIAR-10981 - CryoET of E. coli prepared with the Waffle Method [1504 multi-frame micrographs composed of 14 frames each in TIFF format]

EMPIAR-10981

CryoET of E. coli prepared with the Waffle Method [1504 multi-frame micrographs composed of 14 frames each in TIFF format]

Publication:

Waffle Method: A general and flexible approach for improving throughput in FIB-milling

Kelley K, Jaroenlak P, Raczkowski, AM, Eng ET, Bhabha G, Potter CS, Carragher B, Noble AJ

bioRxiv (2020)

DOI: 10.1101/2020.10.28.359372

Related EMDB entry:

EMD-26396

Deposited:

2022-03-09

Released:

2022-03-21

Last modified:

2022-03-21

Imageset size:

94.00 GB

Imageset DOI:

10.6019/EMPIAR-10981

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs for each tilt image

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1504

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(2.077, 2.077)

Details:

Unaligned K3 tif tilt image frame stacks. Frame number increases from 14 at 0 degrees to 23 at high tilts to account for increasing thickness; dose increases from 4.87 to 8.00 e/A^2. Collected bi-directionally from [0:53.2] then [0:-53.2] with 2.8 degree increments.
Gain references are in the references/ folder.

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

ダウンロードするファイル数やサイズによっては、膨大な時間がかかります。また、この機能は不安定で正常に動作しない可能性があり、ダウンロードされたファイルのチェックサムは検証されません。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）