EMPIAR-10824 - Cryo electron micrographs of Pol delta-DNA-PCNA giving rise to various PCNA tilt angles [multiple data sets in MRC format]

EMPIAR-10824

Cryo electron micrographs of Pol delta-DNA-PCNA giving rise to various PCNA tilt angles [multiple data sets in MRC format]

Publication:

Structure of the processive human Pol δ holoenzyme

Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A

Nature communications 11 (2020)

PMID: 32111820

DOI: 10.1038/s41467-020-14898-6

Related EMDB entry:

EMD-10081 EMD-10080 EMD-10082

Deposited:

2021-09-15

Released:

2021-10-26

Last modified:

2021-10-26

Imageset size:

3.65 TB

Imageset DOI:

10.6019/EMPIAR-10824

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 2

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1406

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.081, 1.081)

Details:

Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.6 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2. Dataset includes 3 corrupted files, as file names suggest - these became corrupted after the deposited EMDB maps were obtained.

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

ダウンロードするファイル数やサイズによっては、膨大な時間がかかります。また、この機能は不安定で正常に動作しない可能性があり、ダウンロードされたファイルのチェックサムは検証されません。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 1

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

452

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.081, 1.081)

Details:

Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.4 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2. Dataset includes 2 corrupted files, as file names suggest - these became corrupted after the deposited EMDB maps were obtained.

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 3

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1717

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.081, 1.081)

Details:

Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.6 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2.

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-17]

324件のPDBエントリーが新たに公開されました。（2026-06-17付）