EMPIAR-10824
Cryo electron micrographs of Pol delta-DNA-PCNA giving rise to various PCNA tilt angles [multiple data sets in MRC format]
Publication:

Structure of the processive human Pol δ holoenzyme

Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A

Nature communications 11 (2020)

PMID: 32111820

Related EMDB entry:
Deposited:
2021-09-15
Released:
2021-10-26
Last modified:
2021-10-26
Imageset size:
3.65 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 2
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1406
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.081, 1.081)
Details:
Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.6 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2. Dataset includes 3 corrupted files, as file names suggest - these became corrupted after the deposited EMDB maps were obtained.
Files:
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2. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 1
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
452
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.081, 1.081)
Details:
Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.4 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2. Dataset includes 2 corrupted files, as file names suggest - these became corrupted after the deposited EMDB maps were obtained.
Files:
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3. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 3
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1717
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.081, 1.081)
Details:
Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.6 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2.
Files:
Loading...
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