EMPIAR-10818 - Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate [3879 multi-frame micrographs composed of 50 frames each in MRC format]

EMPIAR-10818

Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate [3879 multi-frame micrographs composed of 50 frames each in MRC format]

Publication:

Structural basis of substrate recognition by a polypeptide processing and secretion transporter

Kieuvongngam V, Olinares PDB, Palillo A, Oldham ML, Chait BT, Chen J

eLife 9 (2020)

PMID: 31934861

DOI: 10.7554/elife.51492

Related EMDB entry:

EMD-21132

Deposited:

2021-09-10

Released:

2021-11-26

Last modified:

2021-11-26

Imageset size:

1.82 TB

Imageset DOI:

10.6019/EMPIAR-10818

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned and uncorrected multiframe movies of PCAT1 bound to its CtA peptide substrate

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3879

Image size:

(7420, 7676)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.545, 0.545)

Details:

The micrographs are the raw 50-frame tif movies collected with a super-resolution pixel size 0.545 Angstrom (1.09 Angstrom after binning). The dataset is separated into two subdirectories. The mrc movies has been compressed to bz2 format. The gain reference files are in a separate directory as the movie datasets

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

ダウンロードするファイル数やサイズによっては、膨大な時間がかかります。また、この機能は不安定で正常に動作しない可能性があり、ダウンロードされたファイルのチェックサムは検証されません。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-24]

379件のPDBエントリーが新たに公開されました。（2026-06-24付）