EMPIAR-10689
Cryo-electron tomography of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD [multiple data sets in MRC and TIFF formats]
Publication:

The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems

Lasker K, Boeynaems S, Lam V, Scholl D, Stainton E, Briner A, Jacquemyn M, Daelemans D, Deniz A, Villa E, Holehouse AS, Gitler AD, Shapiro L

Nature communications 13 (2022)

PMID: 36163138

Related EMDB entry:
Deposited:
2021-04-07
Released:
2023-01-16
Last modified:
2023-01-16
Imageset size:
5.92 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
  • micrographs - multiframe
1. Unaligned tilt series of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
1
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(4.265, 4.265)
Details:
Tilt-series is motion-corrected using Warp. This tilt-series was generated with the first three frames of each tilt micrograph removed.
Files:
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2. Multi-frame micrographs of each tilt for tilt-series of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
51
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(4.265, 4.265)
Details:
Dark-subtracted frames from serialEM. mdoc metadata file and counting mode gain-reference is included in the same directory.
Files:
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Files:
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Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)