EMPIAR-10675
The Myosin II Coiled Coil Domain Atomic Structure in its Native Environment [multiple data sets in MRC and MRCS formats]
Publication:

The myosin II coiled-coil domain atomic structure in its native environment

Rahmani H, Ma W, Hu Z, Daneshparvar N, Taylor DW, McCammon JA, Irving TC, Edwards RJ, Taylor KA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 33782130

Related EMDB entry:
Deposited:
2019-11-04
Released:
2021-07-29
Last modified:
2022-02-28
Imageset size:
13.30 TB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (Frames*/*/rawdata/*frames.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   16,376,925,619 bytes
Compressed size: 10,465,372,567 bytes
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - unprocessed
1. Frame Stacks 1/3
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1432
Image size:
(8192, 8192)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.009, 1.009)
Details:
Particle positions are given in the final star file in the particle stack directory.
Files:
Loading...
2. Frame Stacks 2/3
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
591
Image size:
(8192, 8192)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.009, 1.009)
Details:
---
Files:
Loading...
3. Frame Stacks 3/3
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1432
Image size:
(8192, 8192)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.009, 1.009)
Details:
---
Files:
Loading...
4. Micrographs before frames alignment
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3507
Image size:
(8192, 8192)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.009, 1.009)
Details:
Micrographs before frame alignment.
Gain References to be used for frame alignment are stored in
data/Micrographs/17dec15_ref_a

directory.
Files:
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5. Micrographs: frame-aligned and dose-weighted
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3507
Image size:
(8192, 8192)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.009, 1.009)
Details:
---
Files:
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6. Particle Stack
Category:
picked particles - single frame - unprocessed
Image format:
MRCS
No. of images or tilt series:
216045
Image size:
(768, 768)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.009, 1.009)
Details:
Particle stack produced using relion with .star and .mrcs files. The mrcs file has been split with the command:
split -b 10000000k 17dec15a-768-localctf.mrcs -d parts-

To combine it back execute:
cat parts-* > 17dec15a-768-localctf_combined.mrcs
Files:
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Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)
Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)
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