EMPIAR-10615
Cryo Soft X-ray data for tetraspeck correlation [multiple data sets in MRC format]
Publication:

Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures

Okolo CA, Kounatidis I, Groen J, Nahas KL, Balint S, Fish TM, Koronfel MA, Cortajarena AL, Dobbie IM, Pereiro E, Harkiolaki M

Nature protocols 16 (2021) 2851-2885

PMID: 33990802

Related EMDB entry:
Deposited:
2021-01-13
Released:
2021-11-01
Last modified:
2022-02-25
Imageset size:
15.07 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
1. Cryo soft X-ray tomography of Tetraspecks on Quantifoil
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(984, 974)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(100.0, 100.0)
Details:
Tetraspeck (100 and 200 nm) on Quantifoil with some 100 nm Au fiducial markers (BBI solutions). hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm
Files:
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2. Cryo Soft X-ray tomography of NIH-3T3 cells with Tetraspeck as correlative fiducial markers
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(984, 974)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(100.0, 100.0)
Details:
Cryo Soft X-ray tomography of NIH-3T3 cells with Tetraspeck (200 nm) as correlative fiducial markers . hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm
Files:
Loading...
3. Cryo Soft X-ray tomography of NIH-3T3 cells for mitochondrial correlation
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
2
Image size:
(984, 974)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(100.0, 100.0)
Details:
Cryo Soft X-ray tomography of NIH-3T3 cells. hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm
Files:
Loading...
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