EMPIAR-10615 - Cryo Soft X-ray data for tetraspeck correlation [multiple data sets in MRC format]

EMPIAR-10615

Cryo Soft X-ray data for tetraspeck correlation [multiple data sets in MRC format]

Publication:

Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures

Okolo CA, Kounatidis I, Groen J, Nahas KL, Balint S, Fish TM, Koronfel MA, Cortajarena AL, Dobbie IM, Pereiro E, Harkiolaki M

Nature protocols 16 (2021) 2851-2885

PMID: 33990802

DOI: 10.1038/s41596-021-00522-4

Related EMDB entry:

Deposited:

2021-01-13

Released:

2021-11-01

Last modified:

2022-02-25

Imageset size:

15.07 GB

Imageset DOI:

10.6019/EMPIAR-10615

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo soft X-ray tomography of Tetraspecks on Quantifoil

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(984, 974)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(100.0, 100.0)

Details:

Tetraspeck (100 and 200 nm) on Quantifoil with some 100 nm Au fiducial markers (BBI solutions). hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Cryo Soft X-ray tomography of NIH-3T3 cells with Tetraspeck as correlative fiducial markers

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(984, 974)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(100.0, 100.0)

Details:

Cryo Soft X-ray tomography of NIH-3T3 cells with Tetraspeck (200 nm) as correlative fiducial markers . hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Cryo Soft X-ray tomography of NIH-3T3 cells for mitochondrial correlation

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(984, 974)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(100.0, 100.0)

Details:

Cryo Soft X-ray tomography of NIH-3T3 cells. hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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