EMPIAR-10604
Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state [1768 multi-frame micrographs composed of 30 frames each in MRCS format]
Publication:

Structural basis for a complex I mutation that blocks pathological ROS production

Yin Z, Burger N, Kula-Alwar D, Aksentijević D, Bridges HR, Prag H, Grba D, Viscomi C, James AJ, Mottahedin A, Kreig T, Murphy MP, Hirst J

Nature communications 12 (2021)

PMID: 33514727

Related EMDB entry:
Deposited:
2020-12-07
Released:
2021-02-12
Last modified:
2021-02-12
Imageset size:
2.75 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
1768
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.35, 1.35)
Details:
Micrographs were imaged using a Gatan K2 summit camera in counting mode and GIF Quantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 15 s exposures captured in 30 frames and a total dose of 50 electrons Å-2
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