EMPIAR-10597 - Human DPP9-CARD8 complex [multiple data sets in TIFF format]

EMPIAR-10597

Human DPP9-CARD8 complex [multiple data sets in TIFF format]

Publication:

Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment

Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H

Immunity 54 (2021) 1392-1404.e10

PMID: 34019797

DOI: 10.1016/j.immuni.2021.04.024

Related EMDB entry:

EMD-22367

Deposited:

2020-12-15

Released:

2021-05-25

Last modified:

2022-02-15

Imageset size:

1.59 TB

Imageset DOI:

10.6019/EMPIAR-10597

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi frame micographs of CARD8-DPP9-noTILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3306

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.825, 0.825)

Details:

For CARD8-DPP9, 3,306 movies were collected at varying defocus levels ranging between −0.8 to −2.2 μm. We used image shift to record two shots for each of the four holes per stage movement. Movies were exposed with a total dose of 58.5 e-/Å2 for 2.22 s fractionated over 49 frames.

Files:

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned multi frame micographs of CARD8-DPP9-apo-TILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2488

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.825, 0.825)

Details:

For CARD8-DPP9, 2,488 movies at a stage tilt of 37° were collected at varying defocus levels ranging between
−1.5 to −3.0 μm. We used image shift to record two shots for each of the four holes per stage movement. Movies were exposed with a total dose of 64.99 e-/Å2 for 2.25 s fractionated over 49 frames for 37° stage tilt movies.

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

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Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-07-01]

288件のPDBエントリーが新たに公開されました。（2026-07-01付）