EMPIAR-10597
Human DPP9-CARD8 complex [multiple data sets in TIFF format]
Publication:

Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment

Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H

Immunity 54 (2021) 1392-1404.e10

PMID: 34019797

Related EMDB entry:
Deposited:
2020-12-15
Released:
2021-05-25
Last modified:
2022-02-15
Imageset size:
1.59 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi frame micographs of CARD8-DPP9-noTILT
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3306
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.825, 0.825)
Details:
For CARD8-DPP9, 3,306 movies were collected at varying defocus levels ranging between −0.8 to −2.2 μm. We used image shift to record two shots for each of the four holes per stage movement. Movies were exposed with a total dose of 58.5 e-/Å2 for 2.22 s fractionated over 49 frames.
Files:
Loading...
2. Unaligned multi frame micographs of CARD8-DPP9-apo-TILT
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2488
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.825, 0.825)
Details:
For CARD8-DPP9, 2,488 movies at a stage tilt of 37° were collected at varying defocus levels ranging between
−1.5 to −3.0 μm. We used image shift to record two shots for each of the four holes per stage movement. Movies were exposed with a total dose of 64.99 e-/Å2 for 2.25 s fractionated over 49 frames for 37° stage tilt movies.
Files:
Loading...
Files:
Loading...
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)