tilt series

MRC

1

(946, 946)

SIGNED 16 BIT INTEGER

(160.0, 160.0)

Tomogram of reovirus-infected U2OS cells 4h post-infection (Area 1)
EMPIAR entry 10418: U2OS_Reo_4hPI_Area1_Reconstruction (S-BIAD20)


Note:
Associated with EMPIAR entry 10419 (adjacent/overlapping FOVs)

Sample characteristics:
Substrate = Quantifoil F1 2/2
Fiducials = Au 250nm nanospheres

Data collection:
Data collected at beamline B24 at Diamond Light source
Data collected 15th Feb 2018
Allocation award ID MX18314

Tilt series:
-60deg to 60deg
1deg step
Total frames 121
1sec exposure

Microscope make:
Zeiss UltraXRM-S220C

Data collection software:
XRM Controller

Microscope setup:
Energy: 500eV
Magnification = x812.5
FOV = 16microns
pixel size = 16x16nm
Zoneplate = 40nm

Data processing:
Type = Automated
Software = Batchruntomo
Alignment protocol = WBP

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