EMPIAR-10418 - Soft X-ray Tomography of mock-infected U2OS cells [1 tilt series in MRC format]

EMPIAR-10418

Soft X-ray Tomography of mock-infected U2OS cells [1 tilt series in MRC format]

Publication:

3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway

Kounatidis I, Stanifer ML, Phillips MA, Paul-Gilloteaux P, Heiligenstein X, Wang H, Okolo CA, Fish TM, Spink MC, Stuart DI, Davis I, Boulant S, Grimes JM, Dobbie IM, Harkiolaki M

Cell 182 (2020) 515-530.e17

PMID: 32610083

DOI: 10.1016/j.cell.2020.05.051

Related EMDB entry:

Deposited:

2020-05-11

Released:

2020-06-30

Last modified:

2022-02-08

Imageset size:

1.17 GB

Imageset DOI:

10.6019/EMPIAR-10418

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tomogram of reovirus-infected U2OS cells 4h post-infection (Area 1)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(946, 946)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(160.0, 160.0)

Details:

Tomogram of reovirus-infected U2OS cells 4h post-infection (Area 1)
EMPIAR entry 10418: U2OS_Reo_4hPI_Area1_Reconstruction (S-BIAD20)

Note:
Associated with EMPIAR entry 10419 (adjacent/overlapping FOVs)

Sample characteristics:
Substrate = Quantifoil F1 2/2
Fiducials = Au 250nm nanospheres

Data collection:
Data collected at beamline B24 at Diamond Light source
Data collected 15th Feb 2018
Allocation award ID MX18314

Tilt series:
-60deg to 60deg
1deg step
Total frames 121
1sec exposure

Microscope make:
Zeiss UltraXRM-S220C

Data collection software:
XRM Controller

Microscope setup:
Energy: 500eV
Magnification = x812.5
FOV = 16microns
pixel size = 16x16nm
Zoneplate = 40nm

Data processing:
Type = Automated
Software = Batchruntomo
Alignment protocol = WBP

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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