EMPIAR-10364
Cryo-electron tomography of E. coli minicells [17 multi-frame micrographs composed of 5 frames each in MRC format]
Publication:

Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain

Burt A, Cassidy CK, Ames P, Bacia-Verloop M, Baulard M, Huard K, Luthey-Schulten Z, Desfosses A, Stansfeld PJ, Margolin W, Parkinson JS, Gutsche I

Nature communications 11 (2020)

PMID: 32029744

Related EMDB entry:
Deposited:
2020-02-06
Released:
2020-04-14
Last modified:
2020-04-14
Imageset size:
141.73 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs from tilt series containing WM4196 minicells
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
17
Image size:
(3838, 3710)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(2.243, 2.243)
Details:
Information on each tilt series in corresponding mdoc files in the mdoc directory.
Data was collected by Daniel Clare on a Titan Krios at eBic, Diamond Light Source, UK.
Each image is taken at ca. 0.2um defocus with a volta phase plate.
Dose per tilt image 1.0 e/A2
Each tilt image is gain corrected

8x binned tomograms with the SIRT-like filter are also provided

This data was used for subtomogram averaging of the in situ chemotaxis receptor array - https://doi.org/10.1038/s41467-020-14350-9
Files:
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