EMPIAR-10364 - Cryo-electron tomography of E. coli minicells [17 multi-frame micrographs composed of 5 frames each in MRC format]

EMPIAR-10364

Cryo-electron tomography of E. coli minicells [17 multi-frame micrographs composed of 5 frames each in MRC format]

Publication:

Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain

Burt A, Cassidy CK, Ames P, Bacia-Verloop M, Baulard M, Huard K, Luthey-Schulten Z, Desfosses A, Stansfeld PJ, Margolin W, Parkinson JS, Gutsche I

Nature communications 11 (2020)

PMID: 32029744

DOI: 10.1038/s41467-020-14350-9

Related EMDB entry:

EMD-10160

Deposited:

2020-02-06

Released:

2020-04-14

Last modified:

2020-04-14

Imageset size:

141.73 GB

Imageset DOI:

10.6019/EMPIAR-10364

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs from tilt series containing WM4196 minicells

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

Image size:

(3838, 3710)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(2.243, 2.243)

Details:

Information on each tilt series in corresponding mdoc files in the mdoc directory.
Data was collected by Daniel Clare on a Titan Krios at eBic, Diamond Light Source, UK.
Each image is taken at ca. 0.2um defocus with a volta phase plate.
Dose per tilt image 1.0 e/A2
Each tilt image is gain corrected

8x binned tomograms with the SIRT-like filter are also provided

This data was used for subtomogram averaging of the in situ chemotaxis receptor array - https://doi.org/10.1038/s41467-020-14350-9

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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