EMPIAR-10270 - Single particle reconstruction of 52 kDa biotin-bound state streptavidin at 3.2 Angstrom resolution [3309 multi-frame micrographs composed of 32 frames each in MRC format]

EMPIAR-10270

Single particle reconstruction of 52 kDa biotin-bound state streptavidin at 3.2 Angstrom resolution [3309 multi-frame micrographs composed of 32 frames each in MRC format]

Publication:

Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW

Nature communications 10 (2019)

PMID: 31160591

DOI: 10.1038/s41467-019-10368-w

Related EMDB entry:

EMD-0689

Deposited:

2019-03-08

Released:

2019-06-07

Last modified:

2019-06-07

Imageset size:

5.49 TB

Imageset DOI:

10.6019/EMPIAR-10270

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Uncorrected biotinbound state streptavidin movie stacks, binning 2 from super-resolution stacks.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3309

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.5265, 0.5265)

Details:

biotin_SA_run_data.star
45686 particles for final 3D reconstruction.

Files:

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Citations

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Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)