EMPIAR-10085
Cryo EM of Type VI Secretion System VipA-N2/VipB contracted sheath [186 multi-frame micrographs composed of 30 frames each in MRC format]
Publication:

Cryo-EM structure of the extended type VI secretion system sheath-tube complex

Wang J, Brackmann M, Castaño-Díez D, Kudryashev M, Goldie KN, Maier T, Stahlberg H, Basler M

Nature microbiology 2 (2017) 1507-1512

PMID: 28947741

Related EMDB entry:
Deposited:
2017-02-20
Released:
2017-07-26
Last modified:
2019-06-07
Imageset size:
79.45 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of T6SS VipA-N2/VipB contracted sheath
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
186
Image size:
(3838, 3710)
Pixel type:
SIGNED BYTE
Pixel spacing:
(1.039, 1.039)
Details:
Data were collected as dark subtracted, non-gain-normalized stacks and saved as LZW-compressed TIFFs (accumulating 30 electron per squared Angstrom in a 9 second exposure).Raw movie stacks were unpacked, converted to mrc and corrected for gain reference with IMOD "clip" command. The micrographs are the mrc movie frames without alignment.
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