All image stacks (accumulating 41 electrons per squared Angstrom exposure in 30 frames / 6 second exposure) were drift-corrected using software UcsfDfCorr. '5 X 5' patch option was used. Dose rate was set to 1.4 electrons to perform the exposure filtering procedure implemented in the program. Deposited data is the sum of all corrected frames.
Particle image stacks extracted from individual micrographs, without further processing. 'particles.star' list all relevant information for every particle in all image stacks (coordinate, defocus etc.)