EMPIAR-11168 - Structure of SARS-CoV-2 membrane protein [multiple data sets in TIFF format]

EMPIAR-11168

Structure of SARS-CoV-2 membrane protein [multiple data sets in TIFF format]

Publication:

Structure of SARS-CoV-2 membrane protein essential for virus assembly

Zhang Z, Nomura N, Muramoto Y, Ekimoto T, Uemura T, Liu K, Yui M, Kono N, Aoki J, Ikeguchi M, Noda T, Iwata S, Ohto U, Shimizu T

Nature communications 13 (2022)

PMID: 35931673

DOI: 10.1038/s41467-022-32019-3

Related EMDB entry:

EMD-31977 EMD-31978 EMD-31976

Deposited:

2022-08-16

Released:

2022-09-09

Last modified:

2022-09-23

Imageset size:

4.37 TB

Imageset DOI:

10.6019/EMPIAR-11168

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. M protein (LMNG/CHS)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6055

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.96 e/Å2/frame
Please use 'CountRef_112-1_0000_Jan07_20.02.08.mrc' as a gain reference without rotation or flipping.
NB: The pixel size in the TIFF header is not correct.
Four digits (0000 to 0008) in the movie file names represent the directions of beam shifts applied during data collection.

Files:

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2. M protein (LMNG/CHS) + Fab-E

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5562

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.97 e/Å2/frame
Please use 'CountCDSRef_ja_139-1_0000_May21_22.27.44.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the movie file names represent the directions of beam shifts applied during data collection.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. M protein (LMNG/CHS) + Fab-B

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3519

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.96 e/Å2/frame
Please use 'CountCDSRef_ja_145-1_0000_May27_23.14.16.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the movie file names represent the directions of beam shifts applied during data collection.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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