EMPIAR-11075 - Single-particle cryo-EM dataset of the Vairimorpha necatrix ribosome [3284 multi-frame micrographs composed of 32 frames each in TIFF format]

EMPIAR-11075

Single-particle cryo-EM dataset of the Vairimorpha necatrix ribosome [3284 multi-frame micrographs composed of 32 frames each in TIFF format]

Publication:

Evolutionary compaction and adaptation visualized by the structure of the dormant microsporidian ribosome

Barandun J, Hunziker M, Vossbrinck CR, Klinge S

Nature microbiology 4 (2019) 1798-1804

PMID: 31332387

DOI: 10.1038/s41564-019-0514-6

Related EMDB entry:

EMD-4935 EMD-4931 EMD-4932 EMD-4933 EMD-4934

Deposited:

2021-09-03

Released:

2022-07-01

Last modified:

2022-07-01

Imageset size:

1.03 TB

Imageset DOI:

10.6019/EMPIAR-11075

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the Vairimorpha necatrix ribosome

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3284

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.6, 0.6)

Details:

Movies with 32 frames were collected at a dose of 8 electrons per pixel per second over an exposure time of 8 seconds. Defocus range of 0.5–4 μm, pixel size of 1.2 Å (0.6 Å super-resolution).

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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