EMPIAR-11046 - Human Xkr8-Basigin complex bound to Fab fragment [1998 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-11046

Human Xkr8-Basigin complex bound to Fab fragment [1998 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

The tertiary structure of the human Xkr8-Basigin complex that scrambles phospholipids at plasma membranes

Sakuragi T, Kanai R, Tsutsumi A, Narita H, Onishi E, Nishino K, Miyazaki T, Baba T, Kosako H, Nakagawa A, Kikkawa M, Toyoshima C, Nagata S

Nature structural & molecular biology 28 (2021) 825-834

PMID: 34625749

DOI: 10.1038/s41594-021-00665-8

Related EMDB entry:

EMD-30636

Deposited:

2022-05-12

Released:

2022-05-27

Last modified:

2022-05-27

Imageset size:

484.76 GB

Imageset DOI:

10.6019/EMPIAR-11046

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned K3 movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1998

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Cs 2.7 mm
- 300 kV
- 1 e/A2/frame

Please use CountCDSRef_134-1_0000_May13_12.33.01.mrc as the gain reference without any rotation and flipping.

Four digits numbers in the movie file names (0000 to 0008) represent the direction of beam-image shifts. We defined nine optics groups in RELION to refine the beam tilt for each group.

final-particles-job094.star.bz2 contains the list (coordinates) of particles used for the final reconstruction deposited in EMDB.

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Citations

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

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Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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