EMPIAR-11046 - Human Xkr8-Basigin complex bound to Fab fragment [1998 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-11046

Human Xkr8-Basigin complex bound to Fab fragment [1998 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

The tertiary structure of the human Xkr8-Basigin complex that scrambles phospholipids at plasma membranes

Sakuragi T, Kanai R, Tsutsumi A, Narita H, Onishi E, Nishino K, Miyazaki T, Baba T, Kosako H, Nakagawa A, Kikkawa M, Toyoshima C, Nagata S

Nature structural & molecular biology 28 (2021) 825-834

PMID: 34625749

DOI: 10.1038/s41594-021-00665-8

Related EMDB entry:

EMD-30636

Deposited:

2022-05-12

Released:

2022-05-27

Last modified:

2022-05-27

Imageset size:

451.47 GB

Imageset DOI:

10.6019/EMPIAR-11046

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned K3 movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1998

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Cs 2.7 mm
- 300 kV
- 1 e/A2/frame

Please use CountCDSRef_134-1_0000_May13_12.33.01.mrc as the gain reference without any rotation and flipping.

Four digits numbers in the movie file names (0000 to 0008) represent the direction of beam-image shifts. We defined nine optics groups in RELION to refine the beam tilt for each group.

final-particles-job094.star.bz2 contains the list (coordinates) of particles used for the final reconstruction deposited in EMDB.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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